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Related Experiment Videos

Medium components adsorbed to mycoplasmal cells.

T Sugiyama

    The Yale Journal of Biology and Medicine
    |September 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Mycoplasma pneumoniae can cause lethal anaphylactic shock in mice when combined with adjuvant. The shock is linked to horse or rabbit serum in the culture medium, not the bacteria itself.

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    Area of Science:

    • Immunology
    • Microbiology

    Background:

    • Mycoplasma pneumoniae is a bacterium known to cause respiratory infections.
    • The potential for Mycoplasma pneumoniae to induce anaphylactic reactions is not well understood.

    Purpose of the Study:

    • To investigate the ability of Mycoplasma pneumoniae to induce lethal anaphylactic shock in a murine model.
    • To identify the component responsible for the anaphylactic reaction.

    Main Methods:

    • Washed Mycoplasma pneumoniae cells were mixed with complete Freund's adjuvant.
    • Injections were administered repeatedly into the foot pad of mice.
    • Mycoplasmas were cultured in media containing either horse serum or rabbit serum.

    Main Results:

    • Repeated injection of Mycoplasma pneumoniae with adjuvant induced lethal anaphylactic shock in mice.

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  • The causative agent was traced to horse serum in the culture medium, and could not be removed by washing.
  • Mycoplasmas grown in rabbit serum also induced a similar effect.
  • No lethal anaphylaxis was observed when Mycoplasma pneumoniae was injected without adjuvant.

    • Conclusions:

    • The lethal anaphylactic shock induced by Mycoplasma pneumoniae in mice is mediated by serum components (horse or rabbit) present in the culture medium, not by the Mycoplasma pneumoniae cells themselves.
    • Complete Freund's adjuvant is essential for eliciting the lethal anaphylactic response.