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Related Experiment Videos

Anticoagulants and erythrocyte filterability.

J Stuart, G S Lucas, N M Caldwell

    Biorheology. Supplement : the Official Journal of the International Society of Biorheology
    |January 1, 1984
    PubMed
    Summary
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    Erythrocyte filterability can be reliably measured after storing whole blood in EDTA or heparin for up to six hours at room temperature. This finding supports using these anticoagulants for blood storage before erythrocyte deformability tests.

    Area of Science:

    • Hematology
    • Biophysics

    Background:

    • Erythrocyte deformability is crucial for microcirculation.
    • Accurate measurement of erythrocyte filterability requires standardized blood storage conditions.

    Purpose of the Study:

    • To evaluate the effect of common anticoagulants (EDTA and heparin) on erythrocyte filterability.
    • To determine the optimal storage duration for whole blood before assessing erythrocyte deformability.

    Main Methods:

    • Erythrocyte deformability was measured using positive-pressure and initial-flow-rate filtration systems (Hémorhéomètre).
    • Whole blood samples were collected in K2EDTA or lithium heparin.
    • Erythrocyte suspensions were prepared after removing contaminating platelets and leucocytes.

    Main Results:

    Related Experiment Videos

    • No significant anticoagulant effect on erythrocyte filtration was observed when platelets and leucocytes were removed.
    • Blood stored in K2EDTA (1.5 mg/ml) or lithium heparin (15 IU/ml) showed no significant change in erythrocyte filterability for up to 6 hours at room temperature.

    Conclusions:

    • K2EDTA and lithium heparin are suitable anticoagulants for short-term blood storage (up to 6 hours) prior to erythrocyte filterability measurements.
    • Contaminating cells (platelets, leucocytes) do not significantly impact erythrocyte filterability measurements when properly removed.