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Artefactual decrease in total protein concentration in patients with monoclonal gammopathies: a method-dependent

K J Whitlow, A A Nanji, M R Pudek

Clinical Biochemistry

|August 1, 1984

Summary

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Monoclonal gammopathy patients

Area of Science:

Clinical Chemistry
Protein Analysis

Background:

Monoclonal gammopathies involve abnormal protein production.
Accurate total protein measurement is crucial for patient management.

Purpose of the Study:

To investigate erroneously low total protein estimates in monoclonal gammopathy patients using the Cobas-Bio analyzer.
To identify the cause of inaccurate readings and propose a solution.

Main Methods:

Utilized the Cobas-Bio centrifugal analyzer for total protein estimation.
Compared results using serum-water blanks versus serum-saline blanks.

Main Results:

Total protein values were erroneously low in monoclonal gammopathy patients with serum-water blanks.
High blank readings were observed, likely due to protein precipitation in low ionic strength conditions.

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Using a serum-saline blank corrected the inaccurate low readings.

Conclusions:

The use of serum-water blanks with the Cobas-Bio analyzer leads to underestimation of total protein in monoclonal gammopathy.
Protein precipitation under low ionic strength conditions is the probable cause.
Employing a serum-saline blank is a reliable method to ensure accurate total protein measurements in these patients.