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Insulin-induced rapid decrease of a major protein in fat cell plasma membranes.

E J Schoenle, L D Adams, D W Sammons

    The Journal of Biological Chemistry
    |October 10, 1984
    PubMed
    Summary
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    Insulin rapidly reduces a key 90 kDa protein in rat fat cell membranes, suggesting its movement is vital for insulin signaling. This discovery enhances understanding of insulin's mechanism of action.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Endocrinology

    Background:

    • Insulin's precise mechanism of action in fat cells remains incompletely understood.
    • Investigating protein concentration changes offers insight into cellular signaling pathways.

    Purpose of the Study:

    • To elucidate the effect of insulin on protein concentrations within rat fat cell fractions.
    • To identify specific proteins involved in insulin signal transduction.

    Main Methods:

    • Utilized two-dimensional electrophoresis and ultrasensitive color silver stain technique.
    • Analyzed protein concentrations in purified rat fat cell plasma membranes and microsomes.

    Main Results:

    • Insulin induced a 90% decrease in a major 90 kDa plasma membrane protein.

    Related Experiment Videos

  • The insulin effect was dose- and time-dependent, with rapid kinetics.
  • Insulin-like growth factor I, orthovanadate, and lanthanum mimicked insulin's effect.
  • Calcium involvement was suggested by experiments with chelating agents and calcium addition.

    • Conclusions:

    • The rapid redistribution of the 90 kDa protein in fat cell plasma membranes is likely integral to insulin signal transmission.
    • This protein's movement may represent a critical step in the overall mechanism of insulin action in adipocytes.