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Sperm surface proteins persist after fertilization.

G G Gundersen, B M Shapiro

    The Journal of Cell Biology
    |October 1, 1984
    PubMed
    Summary
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    Sperm components transferred during fertilization persist in sea urchin embryos, with some proteins remaining intact or partially degraded throughout development. This study investigates the nature and fate of these persistent sperm molecules.

    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Reproductive Biology

    Background:

    • Sperm components are transferred to the egg during fertilization.
    • These components can persist in the early embryo.
    • The nature and fate of these persistent molecules require characterization.

    Purpose of the Study:

    • To characterize the labeled sperm components in sea urchin sperm.
    • To determine if labeled sperm polypeptides remain intact in the embryo after fertilization.
    • To investigate the persistence of sperm components until the pluteus larval stage.

    Main Methods:

    • Labeling sperm with fluorescein isothiocyanate (FITC) or 125I-diiodofluorescein isothiocyanate (125IFC).
    • Using image intensification to observe fluorescent sperm components in embryos.

    Related Experiment Videos

  • Recovering labeled sperm proteins from embryos using an anti-IFC immunoadsorbent.

    • Main Results:

    • Over 60% of sea urchin embryos showed localized fluorescent sperm components at the pluteus larval stage.
    • Approximately 10% of 125IFC in sperm was associated with surface polypeptides.
    • Distinct sets of labeled polypeptides were recovered from both one-cell and gastrula stage embryos, with some retaining original SDS gel mobilities.

    Conclusions:

    • Individual sperm proteins are transferred to the egg at fertilization.
    • Some sperm proteins persist intact or undergo limited degradation in the embryo.
    • These persistent sperm components are detectable until at least the late gastrula stage.