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Adenosine release from stimulated mast cells.

D L Marquardt, H E Gruber, S I Wasserman

    Proceedings of the National Academy of Sciences of the United States of America
    |October 1, 1984
    PubMed
    Summary
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    Reflections.

    Journal of general internal medicine·2016

    Mast cells release adenosine when stimulated, potentially amplifying allergic reactions. This study shows adenosine production from stimulated mast cells, suggesting a positive feedback loop in hypersensitivity.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Adenosine release is observed in lung tissue under hypoxia or antigen exposure.
    • Exogenous adenosine enhances mediator release from stimulated mast cells.

    Purpose of the Study:

    • To investigate adenosine production and release from stimulated mast cells.
    • To explore the role of endogenous adenosine in mast cell-mediated hypersensitivity reactions.

    Main Methods:

    • Purified rat serosal mast cells were sensitized and challenged with antigen, calcium ionophore A23187, or compound 48/80.
    • Adenosine and ATP levels in supernatants and pellets were quantified using high-performance liquid chromatography.
    • Similar experiments were conducted with cultured mouse bone marrow-derived mast cells.

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    Main Results:

    • Stimulated mast cells showed increased extracellular adenosine concentrations.
    • Adenosine levels peaked by 60 seconds post-stimulation, particularly after ionophore challenge.
    • A decline in ATP levels suggested adenosine originates from ATP breakdown.
    • Both rat and mouse mast cells exhibited augmented extracellular adenosine.

    Conclusions:

    • Mast cells produce and release adenosine upon stimulation.
    • Endogenous adenosine may potentiate immediate hypersensitivity reactions via a positive feedback mechanism.