Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pathophysiologic modulation of arachidonate metabolism.

M A Warso, W E Lands

    Clinical Physiology and Biochemistry
    |January 1, 1984
    PubMed
    Summary

    Accurate measurement of lipid hydroperoxides is crucial for understanding health. A new assay reveals normal plasma levels are much lower than previously thought, impacting vascular health.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A first-in-class, first-in-human, phase I trial of p28, a non-HDM2-mediated peptide inhibitor of p53 ubiquitination in patients with advanced solid tumours.

    British journal of cancer·2013
    Same author

    Peptide signaling paths related to intoxication, memory and addiction.

    Addiction biology·2010
    Same author

    Primary prevention in cardiovascular disease: moving out of the shadows of the truth about death.

    Nutrition, metabolism, and cardiovascular diseases : NMCD·2003
    Same author

    Impact of daily food choices on health promotion and disease prevention.

    World review of nutrition and dietetics·2002
    Same author

    Prognostic significance of periodic acid-Schiff-positive patterns in primary cutaneous melanoma.

    Clinical cancer research : an official journal of the American Association for Cancer Research·2001
    Same author

    Commentary on the workshop statement. Essentiality of and recommended dietary intakes for Omega-6 and Omega-3 fatty acids.

    Prostaglandins, leukotrienes, and essential fatty acids·2000

    Area of Science:

    • Biochemistry
    • Physiology
    • Oxidative Stress Research

    Background:

    • Lipid hydroperoxides regulate prostaglandin biosynthesis, but their precise physiological concentrations are debated.
    • Existing assays, like the thiobarbituric acid (TBA) assay, overestimate plasma lipid hydroperoxide levels.
    • High TBA-indicated levels conflict with vascular health, suggesting assay limitations.

    Purpose of the Study:

    • To develop and validate a new, direct assay for quantifying plasma lipid hydroperoxide levels.
    • To resolve the discrepancy between TBA assay results and the physiological requirements for vascular health.
    • To establish accurate normal ranges for plasma lipid hydroperoxides and assess their physiological implications.

    Main Methods:

    • Development of a novel assay for direct measurement of lipid hydroperoxides.
    • Comparative analysis of the new assay against the traditional TBA assay using normal human plasma.
    • Controlled studies to assess assay accuracy and reliability.

    Main Results:

    • The TBA assay significantly overestimates lipid hydroperoxide levels in human plasma (50-100 times higher than direct measurements).
    • The new direct assay indicates normal plasma lipid hydroperoxide levels are approximately 0.5 microM.
    • This lower physiological level suggests a potential chronic stress on endothelial prostacyclin production.

    Conclusions:

    • The TBA assay is unreliable for determining physiological lipid hydroperoxide concentrations in plasma.
    • Normal plasma lipid hydroperoxide levels are substantially lower than previously estimated.
    • Accurate monitoring of plasma hydroperoxides is essential for assessing vascular health and potential risks.

    Related Experiment Videos