Platelet-activating factor stimulates phospholipase in quiescent Swiss mouse 3T3 fibroblast

Platelet-activating factor(PAF) induced the rapid hydrolysis of phospholipids in Swiss mouse 3T3 fibroblasts prelabeled with [3H]arachidonic acid or [3H]choline. Up to 23% of the [3H]arachidonic acid incorporated in phospholipids was released into the medium when PAF was added to cells. We also observed increases of diacylglycerol and lysophosphatidylcholine in the medium and cells after the addition of PAF. These results support the possibility that platelet-activating factor stimulates phospholipase A2 and C activities in 3T3 cells.