Effects of ascorbic acid and disodium edetate on the stability of isoprenaline hydrochloride injection

Disodium edetate (0.01%), either alone or in combination with ascorbic acid (0.1%) was an effective stabilizer for isoprenaline injection B.P. but adjustment to pH 2.8 was necessary. The bubbling of nitrogen through the solution in the ampoule before sealing did not prevent degradation in unprotected solutions. Solutions sterilized by filtration or by autoclaving for 30 min at 116 degrees C were stable for at least 1 year at 5 degrees C or 25 degrees C.