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Related Experiment Videos

Human lymphocyte aggregation?

M A Lazzari, M A Schattner, M Finiasz

    Prostaglandins, Leukotrienes, and Medicine
    |September 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

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    Lymphocyte aggregation was induced by arachidonic acid (AA) and inhibited by aspirin, suggesting cyclo-oxygenase involvement. Other pathways and agents did not induce significant lymphocyte aggregation.

    Area of Science:

    • Immunology
    • Biochemistry
    • Cell Biology

    Background:

    • Lymphocyte aggregation plays a role in immune responses.
    • Understanding the signaling pathways involved in lymphocyte activation is crucial.

    Purpose of the Study:

    • To investigate the mechanisms of lymphocyte aggregation.
    • To identify specific agonists and pathways that induce lymphocyte aggregation.

    Main Methods:

    • Mononuclear cells were isolated using Ficoll-Hypaque gradients.
    • Lymphocyte aggregation was measured using a turbidimetric method.
    • Cells were stimulated with various agents including arachidonic acid, collagen, ADP, and epinephrine.

    Main Results:

    • Arachidonic acid (AA) induced complete and irreversible lymphocyte aggregation.

    Related Experiment Videos

  • Aspirin significantly inhibited AA-induced aggregation, while the lipoxygenase inhibitor ETYA did not.
  • Leukotrienes (LTB4, LTC4, LTD4) and ionophore A23187 did not induce aggregation.
  • TXA2-like material induced aggregation in aspirin-treated lymphocytes.

    • Conclusions:

    • The cyclo-oxygenase pathway, likely via thromboxane A2 (TXA2), is the primary mechanism for AA-induced lymphocyte aggregation.
    • Lymphocytes do not appear to aggregate in response to leukotrienes or direct calcium ionophore stimulation under these conditions.