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[Fluorescence decay curves under pulsed excitation: distinction between free and bound NAD(P)H on isolated living
Abstract:
Under pulsed excitation the decay curve of the intrinsic cellular fluorescence has been recorded using an original equipment. Cell by cell experiments are feasible so that the NAD(P)H bound/free ratio and the relaxation time characteristic of bound NAD(P)H can be used in order to build up histograms characterizing the cell population.