Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simplified urinary oxalate determination using an enzyme electrode.

P Vadgama, W Sheldon, J M Guy

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |September 29, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    High-Intensity Exercise After Percutaneous Coronary Intervention in Previously Physically Active Patients: One-Year Clinical Outcomes.

    Scandinavian journal of medicine & science in sports·2025
    Same author

    In-vivo quantification of lactate using Near Infrared reflectance spectroscopy.

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2021
    Same author

    An electrochemical study of acrylate bone adhesive permeability and selectivity change during <i>in vitro</i> ageing: A model approach to the study of biomaterials and membrane barriers.

    Analytica chimica acta: X·2020
    Same author

    Near Infrared and Aquaphotomic analysis of water absorption in lactate containing media.

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2020
    Same author

    In vitro quantification of lactate in Phosphate Buffer Saline (PBS) samples.

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2020
    Same author

    Comparison of a Genetic Algorithm Variable Selection and Interval Partial Least Squares for quantitative analysis of lactate in PBS.

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2020
    Same journal

    Reference intervals for venous blood gas measurement in a healthy Chinese population.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Multiplex methylation marker analysis for ctDNA detection in liquid biopsies from anal cancer patients: an HPV-independent approach.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Development and validation of patient-based exponentially weighted moving average quality control models for three antipsychotic drugs and their metabolites by LC-MS/MS.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Comparing conventional correction formulas and machine learning-based prediction of ionized calcium.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Micro- and nanoplastics as emerging clinical analytes: analytical validation, interpretive uncertainty, and laboratory actionability in human specimens.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Performance of Free Light Chain reagents in the Dutch External Quality Assessment programme over the past 14 years.

    Clinica chimica acta; international journal of clinical chemistry·2026
    See all related articles

    A new enzyme electrode accurately measures urine oxalate levels. This biosensor, using immobilized oxalate decarboxylase and a CO2 sensor, offers a reliable method for clinical diagnostics.

    Area of Science:

    • Biomedical Engineering
    • Analytical Chemistry
    • Enzyme Technology

    Background:

    • Oxalate is a key metabolite in urine, and its accurate measurement is crucial for diagnosing conditions like kidney stones.
    • Existing methods for urine oxalate determination can be complex or time-consuming.
    • Development of rapid and reliable biosensors for clinical analysis is an ongoing area of research.

    Purpose of the Study:

    • To develop and evaluate a novel enzyme electrode for the quantitative measurement of oxalate in urine.
    • To assess the performance characteristics of the developed biosensor, including its linear range, sensitivity, and stability.
    • To compare the results obtained from the enzyme electrode with a standard colorimetric method.

    Main Methods:

    • An enzyme electrode was constructed by entrapping oxalate decarboxylase in acrylamide gel and immobilizing it over a carbon dioxide (CO2) sensor.

    Related Experiment Videos

  • Urine samples were pre-treated with ethylenediaminetetraacetic acid (EDTA).
  • The electrode's performance was evaluated using standard oxalate solutions and clinical urine specimens, with comparisons made to a colorimetric assay.

    • Main Results:

    • The enzyme electrode demonstrated a linear response for urine oxalate concentrations in the range of 0-0.2 mmol/l.
    • Urine pre-treatment with EDTA was required, but oxalate extraction was unnecessary.
    • The biosensor showed good stability, with inhibition by phosphate and sulfate only apparent after 10-14 days.
    • Analysis of 50 urine specimens showed a strong correlation (r = 0.955) with a widely-used colorimetric method.

    Conclusions:

    • The developed enzyme electrode provides a sensitive and reliable method for urine oxalate determination.
    • This biosensor offers a viable alternative to traditional methods, potentially simplifying clinical diagnostics.
    • The electrode exhibits good operational stability and minimal interference from common urine components, making it suitable for routine analysis.