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Paul-Bunnell antigen in normal human tissues.

K Kano, T Tamura, F Milgrom

    Diagnostic Immunology
    |January 1, 1984
    PubMed
    Summary
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    The BS antigen, part of the Paul-Bunnell (P-B) complex, was found in healthy individuals' leukocytes and kidney tissues. This antigen may shift from intracellular to cell-surface expression in certain diseases.

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • The Paul-Bunnell (P-B) antigen complex includes the BS antigen, shared by bovine (BRBC) and sheep red blood cells (SRBC).
    • Infectious mononucleosis (IM) is associated with specific antibody responses to P-B antigens.

    Purpose of the Study:

    • To investigate the presence and expression of the BS antigen in healthy individuals and its potential role in disease.
    • To characterize the BS antigen's localization (intracellular vs. cell-surface) and form (soluble vs. bound).

    Main Methods:

    • Chloroform-methanol extraction of peripheral blood leukocytes.
    • Precipitation assays using infectious mononucleosis (IM) sera.
    • Mixed agglutination tests with bovine red blood cells (BRBC) and kidney tissue sediments.

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    Main Results:

    • The BS antigen was detected in leukocyte extracts from 4 of 26 healthy individuals.
    • An extract containing the BS antigen formed a precipitation line with IM sera, identical to purified P-B antigen from BRBC.
    • The BS antigen was also identified in normal human kidney tissue using mixed agglutination.

    Conclusions:

    • The BS antigen is present in normal human tissues, potentially as an intracellular antigen in healthy individuals.
    • Pathologic conditions may lead to the expression of the BS antigen on cell surfaces or its release into circulation as a soluble form.