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Transcription and translation dependent induction of interleukin 2 (IL-2) and IL-2 receptors
Our previous findings on the expression of Interleukin 2 (IL-2) receptors have shown that under the influence of certain drugs, it was possible to dissociate IL-2 production from the appearance of IL-2 receptors on human peripheral blood lymphocytes (PBL). Therefore, we studied whether the induction of IL-2 receptors were transcription- or translation-dependent. Actinomycin D and puromycin inhibited the appearance of IL-2 receptors as well as the production of IL-2. Furthermore, both drugs also inhibited human PBL to enter the G1 phase of the cell cycle. Thus, these data suggested that both IL-2 production and the appearance of IL-2 receptors were dependent on transcription and translation in non-resting cells.
Our previous findings on the expression of Interleukin 2 (IL-2) receptors have shown that under the influence of certain drugs, it was possible to dissociate IL-2 production from the appearance of IL-2 receptors on human peripheral blood lymphocytes (PBL). Therefore, we studied whether the induction of IL-2 receptors were transcription- or translation-dependent. Actinomycin D and puromycin inhibited the appearance of IL-2 receptors as well as the production of IL-2. Furthermore, both drugs also inhibited human PBL to enter the G1 phase of the cell cycle. Thus, these data suggested that both IL-2 production and the appearance of IL-2 receptors were dependent on transcription and translation in non-resting cells.