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Pristinamycin accumulation by Staphylococcus aureus.

P Lacroix, M L Capmau, F Le Goffic

    The Journal of Antibiotics
    |October 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

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    Pristinamycins accumulate rapidly in Staphylococcus aureus, reaching high internal concentrations. This accumulation is likely due to passive diffusion and binding to bacterial ribosomes, not synergistic activity.

    Area of Science:

    • Microbiology
    • Pharmacology
    • Biochemistry

    Background:

    • Staphylococcus aureus is a significant human pathogen.
    • Pristinamycins are antibiotics with known synergistic activity.
    • Understanding antibiotic accumulation is crucial for combating bacterial resistance.

    Purpose of the Study:

    • To investigate the accumulation of pristinamycins IA and IIA (PIA and PIIA) in Staphylococcus aureus.
    • To explore the mechanism of antibiotic entry and concentration within bacterial cells.
    • To assess the role of synergistic activity at the accumulation level.

    Main Methods:

    • Studied accumulation of pristinamycins IA and IIA using hydrogenated analogs (H2)PIA and (H2)PIIA.
    • Incubated Staphylococcus aureus with antibiotics at 37°C.

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  • Tested the effect of metabolic inhibitors and tetracycline on antibiotic accumulation.

    • Main Results:

    • Observed rapid accumulation of both (H2)PIA and (H2)PIIA in Staphylococcus aureus.
    • Internal antibiotic concentrations reached up to 58-fold the external concentration.
    • Accumulation was not reduced by metabolic inhibitors or tetracycline, and synergistic activity was not observed at the accumulation level.

    Conclusions:

    • Pristinamycins likely enter Staphylococcus aureus via passive diffusion.
    • Internal antibiotic concentration is maintained by binding to bacterial ribosomes.
    • Synergistic activity of pristinamycins does not appear to be a factor in their bacterial accumulation.