Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

B- and T-cell determination in ALL.

H Winterleitner, W Knapp

    Haematologia
    |January 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Cytostatic treatments for childhood leukemia (ALL) alter B-cell percentages but not T-cells. Lymphoid cell behavior varies before treatment, with potential implications for understanding immune responses in ALL patients.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Oecologia·2017
    Same author

    Simultaneous Phenotypic and Genotypic Studies of Individual Mitotic Cells-Methods and Practical Applications.

    Leukemia & lymphoma·2016
    Same author

    Culture and animal experiments in tuberculosis diagnosis.

    Rontgenphotographie; medizinsche Photographie und medizinische Laboratoriumspraxis·2014
    Same author

    Dendritic Cell Generation from Highly Purified CD14+Monocytes.

    Methods in molecular medicine·2011
    Same author

    CD Antigens 1989: Summary of Nomenclature System for Human Leacocyte Surface Antigens.

    The American journal of pathology·2009
    Same author

    Near-tetraploid acute myeloid leukemias: an EGIL retrospective study of 25 cases.

    Leukemia·2006
    Same journal

    The use of granulocyte colony-stimulating factor in a neutropenic renal transplant recipient.

    Haematologia·2003
    Same journal

    Heterozygous methylene tetrahydrofolate reductase mutation with mild hyperhomocysteinemia associated with deep vein thrombosis.

    Haematologia·2003
    Same journal

    Congenital methemoglobinemia due to NADH-methemoglobin reductase deficiency in three Indian families.

    Haematologia·2003
    Same journal

    Angiocentric lymphomatoid granulomatosis and severe hypogammaglobulinaemia.

    Haematologia·2003
    Same journal

    Vena cava superior syndrome due to sternal plasmacytoma in the course of systemic myeloma.

    Haematologia·2003
    Same journal

    Hepatosplenic gamma delta T-cell lymphoma with leukemic phase successfully treated with 2-chlorodeoxyadenosine.

    Haematologia·2003
    See all related articles

    Area of Science:

    • Immunology
    • Pediatric Oncology
    • Cell Biology

    Background:

    • Childhood acute lymphoblastic leukemia (ALL) and undifferentiated leukemia (UAL) involve complex immune system alterations.
    • Understanding the impact of cytostatic therapies on lymphocyte subsets is crucial for treatment monitoring and prognosis.

    Purpose of the Study:

    • To investigate the effects of various cytostatic treatment regimens on T-cell and B-cell behavior in children diagnosed with ALL and UAL.
    • To characterize the distinct patterns of lymphoid cell behavior observed in the initial phase of ALL before therapeutic intervention.

    Main Methods:

    • Flow cytometry analysis to quantify T-cell and B-cell percentages.
    • Assessment of lymphoid cells for membrane-bound immunoglobulin (Ig) and rosette formation.

    Related Experiment Videos

  • Evaluation of cell membrane fluidity, capping, and pitching behaviors.

    • Main Results:

    • Cytostatic treatments led to a decrease in B-cell percentages, while T-cell percentages remained within normal ranges.
    • In long-term remission, lymphocyte values normalized.
    • Pre-treatment ALL patients exhibited varied lymphoid cell profiles: some lacked membrane-bound Ig and rosette formation, others predominantly showed T-cells, and a third group had diminished rosette formation despite normal Ig levels.
    • Cell membrane fluidity was comparable between patients and healthy controls.

    Conclusions:

    • Cytostatic therapy significantly impacts B-cell populations in pediatric leukemia, with T-cells showing resilience.
    • Pre-treatment lymphoid cell heterogeneity in ALL suggests distinct immunological states that warrant further investigation.
    • The role of membrane-bound Ig, potentially including blocking antibodies, requires further clarification in the context of ALL pathogenesis.