Related Experiment Video
Updated: Aug 17, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
Changes in the character of Factor VIII inhibitors following Factor VIII transfusion
Abstract:
Factor VIII inhibitors from 4 haemophiliacs have been studied at intervals following transfusion of a factor VIII preparation with an aim to correlate their reaction kinetic and avidity with their immuno-chemical character. Post-treatment antibodies are more avid than pre-treatment antibodies and show a second order reaction while pre-treatment antibodies produce a graph of complex nature for the time course inactivation of Factor VIII. It seems that IgG3 anti VIII:C that forms a significant part of post-treatment antibody is responsible for these discrepancies.
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