Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure-function relationships of human factor VIII complex studied by thioredoxin dependent disulfide reduction.

B Hessel, H Jörnvall, L Thorell

    Thrombosis Research
    |September 15, 1984
    PubMed
    Summary

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Characterization of 3-Dimensional Printing and Casting Materials for use in Computed Tomography and X-ray Imaging Phantoms.

    Journal of research of the National Institute of Standards and Technology·2024
    Same author

    Relationship Between Postmortem Urine and Blood Concentrations of GHB Furnishes Useful Information to Help Interpret Drug Intoxication Deaths.

    Journal of analytical toxicology·2018
    Same author

    Genetic variations in the annexin A5 gene and the risk of pregnancy-related venous thrombosis.

    Journal of thrombosis and haemostasis : JTH·2014
    Same author

    Adolescent growth: genes, hormones and the peer group. Proceedings of the 20th Aschauer Soiree, held at Glücksburg castle, Germany, 15th to 17th November 2013.

    Pediatric endocrinology reviews : PER·2014
    Same author

    Concentration ratios of methamphetamine to amphetamine in blood can help to distinguish use of methamphetamine from various mixtures of the two stimulants.

    Journal of analytical toxicology·2012
    Same author

    In vivo redox state of human thioredoxin and redox shift by the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA).

    Free radical biology & medicine·2012

    Redox systems, like thioredoxin, can regulate factor VIII (coagulation factor) activity. Reducing disulfide bonds in factor VIII complexes alters its structure and function, impacting hemostasis.

    Area of Science:

    • Biochemistry
    • Hematology
    • Molecular Biology

    Background:

    • Factor VIII complex, comprising factor VIII: von Willebrand factor (vWF) and factor VIII: C (coagulation factor), is crucial for hemostasis.
    • The complex contains numerous disulfide bonds, suggesting a role for redox regulation.

    Purpose of the Study:

    • To investigate the impact of disulfide bond reduction on the factor VIII complex structure and function.
    • To explore the potential role of redox systems, specifically the thioredoxin system, in regulating factor VIII activity.

    Main Methods:

    • Purification of a multimeric factor VIII complex.
    • Limited and prolonged reduction of disulfide bonds using NADPH, thioredoxin reductase, and thioredoxin.
    • Analysis of complex disaggregation, subunit appearance, and functional activity (platelet agglutination).

    Related Experiment Videos

  • N-terminal amino acid sequencing and identification of key cysteine residues.

    • Main Results:

    • Limited reduction caused partial disaggregation of factor VIII:vWF multimers, loss of ristocetin-induced platelet agglutination, and dissociation of factor VIII:C.
    • No Mr 260,000 VIII:vWF subunits were observed after limited reduction.
    • Prolonged reduction led to the reappearance of different multimers and Mr 260,000 subunits.
    • Two cysteine residues in the N-terminus of VIII:vWF were identified as critical for thioredoxin interaction.

    Conclusions:

    • The thioredoxin system and other redox systems may regulate factor VIII activity.
    • Disulfide bond reduction significantly alters factor VIII complex structure and function.
    • Redox regulation could play a role in vivo hemostatic processes.