Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chlorpromazine: a potential anticancer agent?

S Darkin, J McQuillan, R K Ralph

    Biochemical and Biophysical Research Communications
    |November 30, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Medically Defined Infertility Versus Self-Perceived Fertility Problem: Implications of Survey Wording for Assessing Associations with Depressive Symptoms.

    Women's health reports (New Rochelle, N.Y.)·2021
    Same author

    Cytokinin control of growth of Spirodela oligorrhiza in darkness.

    Planta·2014
    Same author

    Neck narrowing in resurfacing hip arthroplasty: a vascular insult?

    Irish journal of medical science·2012
    Same author

    Computer-aided detection for CT colonography: incremental benefit of observer training.

    The British journal of radiology·2008
    Same author

    Psychological distress by type of fertility barrier.

    Human reproduction (Oxford, England)·2006
    Same author

    Conventional and experimental treatment of cerebral malaria.

    International journal for parasitology·2006

    The antipsychotic drug chlorpromazine breaks DNA in mouse cells, which reseals upon drug removal. This suggests chlorpromazine may interfere with topoisomerase, offering potential as an anti-cancer agent.

    Area of Science:

    • Molecular biology
    • Pharmacology
    • Cancer research

    Background:

    • Antipsychotic drugs can exhibit unexpected biological activities.
    • DNA topoisomerases are crucial for DNA replication and are targets for anti-cancer drugs.
    • Chlorpromazine is a well-known antipsychotic medication.

    Purpose of the Study:

    • To investigate the effect of chlorpromazine on DNA integrity in PY815 mouse mastocytoma cells.
    • To explore the potential mechanism of chlorpromazine's interaction with DNA, particularly its effect on topoisomerase.
    • To assess the potential of chlorpromazine as an anti-cancer agent.

    Main Methods:

    • Treatment of PY815 mouse mastocytoma cells and isolated nuclei with chlorpromazine.
    • Observation of DNA scission and resealing.

    Related Experiment Videos

  • Analysis of protein association with broken DNA fragments.

    • Main Results:

    • Chlorpromazine induced DNA scission in both intact cells and isolated nuclei.
    • The broken DNA resealed upon removal of chlorpromazine from isolated nuclei.
    • Unlike other DNA-intercalating anti-cancer drugs, no protein was associated with the broken DNA after chlorpromazine treatment.

    Conclusions:

    • Chlorpromazine's DNA-breaking and resealing properties suggest interference with topoisomerase activity.
    • The lack of protein association indicates a potentially novel mechanism of topoisomerase inhibition by chlorpromazine.
    • Chlorpromazine warrants further investigation for its potential anti-cancer therapeutic applications.