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Electrophoretic mobility of mouse immune (gamma) interferon produced by a cloned T-cell lymphoma
In the present study we have analyzed the electrophoretic mobility of murine IFN-gamma produced by a T-cell lymphoma upon stimulation with phorbol myristic acetate. Two peaks of antiviral activity were recovered after gel electrophoresis of partially purified MuIFN-gamma, in the regions of 30 kd and 43 kd. Since MuIFN-gamma displays a molecular weight of about 16-17 kd in denaturating conditions, the above values represent oligomeric forms, probably composed of two or three units of lower molecular weight.
In the present study we have analyzed the electrophoretic mobility of murine IFN-gamma produced by a T-cell lymphoma upon stimulation with phorbol myristic acetate. Two peaks of antiviral activity were recovered after gel electrophoresis of partially purified MuIFN-gamma, in the regions of 30 kd and 43 kd. Since MuIFN-gamma displays a molecular weight of about 16-17 kd in denaturating conditions, the above values represent oligomeric forms, probably composed of two or three units of lower molecular weight.