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Updated: Aug 1, 2026

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
Published on: October 25, 2016
Primary structure of human T-cell receptor alpha-chain
Researchers identified cDNA clones for the T-cell receptor alpha-chain, revealing its structure and gene rearrangement. This finding advances understanding of T-cell recognition and its link to immunoglobulins.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- The T-cell receptor (TCR) mediates major histocompatibility complex (MHC)-restricted antigen recognition.
- TCRs are composed of disulfide-linked alpha and beta glycopeptides, structurally similar to immunoglobulins.
Purpose of the Study:
- To identify cDNA clones encoding the alpha-chain of the human T-cell receptor.
- To investigate the molecular basis of TCR-mediated antigen recognition.
Main Methods:
- Utilized anti-TCR monoclonal antibodies to isolate and characterize TCRs from human and murine T-cell clones.
- Employed molecular cloning techniques, including cDNA probes, to analyze TCR alpha-chain gene expression and rearrangement.
Main Results:
- Isolated and characterized cDNA clones encoding the alpha-chain of the human T-cell receptor from the HPB-MLT cell line.
- Demonstrated that alpha-chain mRNA expression and V-gene segment rearrangement occur exclusively in T cells.
- The predicted protein sequence of the alpha-chain shows homology to TCR beta-chains and immunoglobulin chains, particularly in variable (V) and joining (J) segments.
Conclusions:
- The alpha-chain of the T-cell receptor shares evolutionary and structural similarities with immunoglobulin chains.
- Gene rearrangement of V-gene segments is crucial for functional T-cell receptor alpha-chain expression in T cells.
- These findings contribute to understanding the molecular mechanisms underlying T-cell recognition and immune responses.
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