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Enzymatic synthesis of anticodon-deleted and replaced Bacillus subtilis tRNAThr and their amino acid acceptor
Nucleic Acids Symposium Series
|January 1, 1984
Abstract:
By some enzymatic and chemical procedures, one or two nucleotides in the anticodon region of Bacillus subtilis tRNAThr were deleted and a part of the anticodon sequence was replaced. The anticodon-deleted molecules retained amino acid acceptor activity although the activity was low. However, the anticodon-replaced molecule which has a lysine specific sequence (U-U-U) exhibited neither threonine acceptor activity nor lysine acceptor activity.