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[Production of an enriched factor VIII reagent]
Folia Haematologica (Leipzig, Germany : 1928)
|January 1, 1976
Summary
Researchers developed an enriched factor VIII preparation from cryoprecipitates using dextran precipitation. Both dextran and poly(ethylene glycol) methods yielded similar results for factor VIII enrichment and purification.
Area of Science:
- Hematology
- Biochemistry
- Protein Chemistry
Background:
- Factor VIII is crucial for blood coagulation.
- Hemophilia A is a bleeding disorder caused by Factor VIII deficiency.
- Cryoprecipitates are a source of therapeutic proteins like Factor VIII.
Purpose of the Study:
- To develop an enriched Factor VIII preparation from cryoprecipitates.
- To compare the efficacy of dextran and poly(ethylene glycol) precipitation methods for Factor VIII purification.
Main Methods:
- Factor VIII was isolated from cryoprecipitates.
- Purification involved two precipitation phases: fibrinogen removal followed by antihemophilic globulin (Factor VIII) removal.
- Dextran and poly(ethylene glycol) were used as precipitating agents for comparison.
Main Results:
- An enriched Factor VIII preparation was successfully produced.
- Both dextran and poly(ethylene glycol) precipitation methods resulted in comparable yields and enrichment levels of Factor VIII.
- The two-phase precipitation effectively removed fibrinogen and other impurities.
Conclusions:
- Dextran precipitation is an effective method for producing enriched Factor VIII concentrates.
- Poly(ethylene glycol) precipitation offers similar efficacy to dextran for Factor VIII purification.
- The developed method provides a viable approach for Factor VIII concentrate production.