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Related Experiment Videos

Artificial red cells with crosslinked hemoglobin membranes.

T A Davis, W J Asher, H W Wallace

    Applied Biochemistry and Biotechnology
    |January 1, 1984
    PubMed
    Summary
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    Researchers created artificial red blood cells using hemoglobin and glutaraldehyde. These artificial cells successfully encapsulated hemoglobin, retaining oxygen-binding capacity and demonstrating potential for oxygen transport applications.

    Area of Science:

    • Biomimetic materials science
    • Artificial cell development
    • Hemoglobin-based oxygen carriers

    Background:

    • Hemoglobin (Hb) is crucial for oxygen transport in red blood cells.
    • Developing artificial oxygen carriers is essential for medical applications.
    • Encapsulating Hb effectively is key to creating functional artificial cells.

    Purpose of the Study:

    • To prepare artificial cells encapsulating concentrated hemoglobin solution.
    • To develop a method for creating artificial red blood cells using interfacial polymerization.
    • To evaluate the oxygen-binding capacity of the prepared artificial cells.

    Main Methods:

    • Artificial cells were prepared via interfacial polymerization of Hb with glutaraldehyde (GA) in liquid membrane capsules (LMC).

    Related Experiment Videos

  • A 30% Hb solution was emulsified in mineral oil, then dispersed in an aqueous GA phase.
  • Surfactants were used to release and suspend cells; agitation controlled cell size.

    • Main Results:

    • Artificial red blood cells encapsulating Hb solution were successfully prepared.
    • The encapsulated Hb retained its capacity to bind and release oxygen.
    • The artificial cells exhibited a P50 of 8.9 torr and an oxygen capacity of 0.55 cc O2/g Hb.

    Conclusions:

    • Interfacial polymerization in LMC is an effective method for creating artificial red blood cells.
    • The artificial cells demonstrate functional oxygen transport capabilities.
    • Crosslinked Hb at the cell surface does not contribute to oxygen transport, but the encapsulated Hb remains functional.