Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TRH immunoreactivity in the thyroid gland.

E Iversen, J Weeke, P Laurberg

    Scandinavian Journal of Clinical and Laboratory Investigation
    |December 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Authors' reply re: Attention deficit hyperactivity disorder and autism spectrum disorder in children born to mothers with thyroid dysfunction: a Danish nationwide cohort study.

    BJOG : an international journal of obstetrics and gynaecology·2016
    Same author

    Hypothyroidism incidence in and around pregnancy: a Danish nationwide study.

    European journal of endocrinology·2016
    Same author

    Vitamin D-rich marine Inuit diet and markers of inflammation - a population-based survey in Greenland.

    Journal of nutritional science·2016
    Same author

    Neuropsychology and brain morphology in Klinefelter syndrome - the impact of genetics.

    Andrology·2014
    Same author

    Attention deficit hyperactivity disorder and autism spectrum disorder in children born to mothers with thyroid dysfunction: a Danish nationwide cohort study.

    BJOG : an international journal of obstetrics and gynaecology·2014
    Same author

    Associations between antibiotic exposure during pregnancy, birth weight and aberrant methylation at imprinted genes among offspring.

    International journal of obesity (2005)·2013

    Thyroid tissue in dogs and pigs contains thyrotropin-releasing hormone (TRH) immunoreactivity. This immunoreactivity behaves similarly to synthetic TRH, suggesting its presence in the thyroid gland.

    Area of Science:

    • Endocrinology
    • Biochemistry
    • Animal Science

    Background:

    • Thyrotropin-releasing hormone (TRH) is primarily known for its role in the hypothalamus-pituitary-thyroid axis.
    • Previous research has not extensively investigated the presence of TRH within thyroid tissue itself.

    Purpose of the Study:

    • To investigate the presence and characteristics of thyrotropin-releasing hormone immunoreactivity (iTRH) in dog and pig thyroid tissue.
    • To determine if the identified iTRH exhibits properties similar to synthetic TRH.

    Main Methods:

    • Methanol extraction of dog and pig thyroid tissue.
    • Characterization of iTRH using gel filtration (Sephadex G-25), thin-layer chromatography, and cation exchange chromatography (SP-Sephadex C-25).
    • Incubation of purified iTRH with rat serum to assess stability.

    Related Experiment Videos

    Main Results:

    • Significant amounts of iTRH were detected in methanol extracts of both dog (2.98 ± 1.38 pmol/g) and pig (1.83 ± 0.58 pmol/g) thyroid tissue.
    • The iTRH was distinct from ether-extractable materials and exhibited chromatographic behavior consistent with synthetic TRH.
    • Cation exchange chromatography revealed heterogeneity, but a portion eluted identically to synthetic TRH.
    • Purified iTRH degraded in parallel with synthetic TRH when incubated with rat serum.

    Conclusions:

    • Thyroid tissue from dogs and pigs contains immunoreactive TRH.
    • The detected iTRH shares biochemical and functional characteristics with synthetic TRH.
    • These findings suggest the thyroid gland may contain endogenous TRH.