von Willebrand Factor/Factor VIII adsorption to surfaces from human plasma

The adsorption of human von Willebrand factor/factor VIII (VWF/VIII) from citrated plasma to a series of polymeric surfaces and glass was measured with both prelabelled 125I VWF/VIII and an 125I antibody to VWF/VIII. Adsorption to the various surfaces differed but was generally quite low (0.24 to 6.5 ng/cm2). Results from both techniques agreed well. The relationship between VWF/VIII adsorption and the thrombogenicity of the surfaces was examined but no clear trend could be discerned. Further studies will therefore be required to determine whether the variation in VWF/VIII adsorption influences the thrombogenicity of the surfaces.