Preparation, assessment and preservation of antigen from Brucella abortus strain 45/20 for use in the rough antigen...

Two methods of extraction were used to prepare antigens from Brucella abortus rough strain 45/20. The antigens were assessed for use in the complement fixation test. A suitable antigen was prepared using the saline extraction method of Miller et al. (1976) and used extensively in CF tests. Four methods of preservation were compared; -20 degrees C, -196 degrees C, 0.5% phenol at 4 degrees C, and lyophilisation. The antigen could be stored at -20 degrees C or -196 degrees C for up to 2 years.