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Related Experiment Videos

Protein III, a neuron-specific phosphoprotein: variant forms found in human brain.

E Perdahl, W C Wu, M D Browning

    Neurobehavioral Toxicology and Teratology
    |November 1, 1984
    PubMed
    Summary

    Human brain Protein III exhibits distinct variants, unlike rat brain. These variants, including Protein IIIa and Protein IIIb, showed varied distribution across neurological conditions.

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    Area of Science:

    • Neuroscience
    • Molecular Biology
    • Biochemistry

    Background:

    • Protein III is a neuron-specific phosphoprotein associated with synaptic vesicles.
    • It comprises two polypeptides: IIIa (74 kD) and IIIb (55 kD).

    Purpose of the Study:

    • To investigate Protein III variants in human brain tissue.
    • To compare human Protein III variants with those found in rat brain.
    • To analyze the distribution of Protein III variants in various neuropathological conditions.

    Main Methods:

    • Biochemical assays
    • Immunochemical assays
    • Electrophoretic mobility analysis
    • Peptide mapping

    Main Results:

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    • Human brain Protein IIIb exists in three variants (55, 57, and 59 kD), differing from the single form found in rat brain.
    • Protein IIIa also displayed three variants in human brain.
    • Peptide mapping confirmed conserved differences in variant forms of a smaller peptide fragment.
    • Observed differences in variant distribution across clinical categories including chronic alcoholism, Alzheimer's disease, multi-infarct dementia, and Parkinson's disease.

    Conclusions:

    • Human brain Protein III exhibits greater molecular heterogeneity than previously reported.
    • The distinct variants of Protein III may have implications for understanding neuropathological conditions.