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Related Experiment Videos

On evolutionarily conserved simple repetitive DNA sequences: do "sex-specific" satellite components serve any

J T Epple1, A Cellini, M Shorte

  • 1City of Hope Research Institute, Duarte, CA 91010.

Differentiation; Research in Biological Diversity
|January 1, 1983
PubMed
Summary
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Simple repetitive DNA sequences are conserved across vertebrates and transcribed into RNA. These sequences are sex-specifically arranged and can help study biological functions like sex determination.

Area of Science:

  • Genomics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Eukaryotic genomes contain repetitive DNA, including simple repetitive sequences (SRSs).
  • The distribution, function, and evolutionary conservation of SRSs are not fully understood.
  • SRSs are found in non-random patterns within genomes.

Purpose of the Study:

  • To investigate the biological role and evolutionary history of SRSs.
  • To analyze the function and arrangement of SRSs in sex-specific snake satellite DNA.
  • To explore the potential of SRSs as tools for studying fundamental biological processes.

Main Methods:

  • Sequence analysis of snake satellite DNA to identify SRSs.
  • Comparative genomics to assess evolutionary conservation across vertebrates.

Related Experiment Videos

  • RNA transcription and translation assays in various animal and bacterial systems.
  • Genomic mapping to determine the interspersion patterns of SRSs with single-copy DNA.
  • Analysis of mRNA in mouse polysomal poly(A)+ RNA.

    • Main Results:

    • Identified the snake SRS as 5'-GATAGACA-3', strictly conserved throughout vertebrate evolution.
    • Found SRSs interspersed with single-copy DNA in the mouse genome.
    • Demonstrated that SRSs are transcribed into RNA and are translatable.
    • Revealed sex-specific arrangement of SRSs in vertebrates.
    • Observed SRSs adjacent to a sequence identifying a male-specific mRNA in mice.

    Conclusions:

    • The conserved SRS (5'-GATAGACA-3') is transcribed and translatable, suggesting potential functional roles.
    • The sex-specific arrangement and association with male-specific mRNA indicate a role in sex-related processes.
    • SRSs, even if not directly involved in sex determination, serve as valuable tools for investigating such biological problems.