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Cryosectioning Yeast Communities for Examining Fluorescence Patterns
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Scanning electron microscopy of yeasts in seborrheic keratoses

W H Wilborn, L F Montes

    Journal of Cutaneous Pathology
    |February 1, 1980
    PubMed

    Abstract:

    Lesions of seborrheic keratoses were studied by scanning electrom microscopy. Numerous yeasts were present on the karatinized cells and hairs associated with the lesions. Surfaces of some yeasts were smooth and others were ruffled. The ruffles suggested the presence of a glycocalyx. The yeasts exhibited the morphological features of Pityrosporum ovale.

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    Scanning Electron Microscopy01:07

    Scanning Electron Microscopy

    A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
    Fundamental Principles
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