Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chicken-gizzard actin: polymerization and stability.

H Strzelecka-Gołaszewska, E Próchniewicz, E Nowak

    European Journal of Biochemistry
    |February 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Estimation of initial stroke severity in hospital databases using NIHSS score from population-based stroke registries.

    Revue neurologique·2026
    Same author

    Ischemic stroke care for patients affiliated to the French agricultural health insurance scheme: A national study.

    Revue neurologique·2025
    Same author

    Venous thromboembolism in Cushing syndrome: results from an EuRRECa and Endo-ERN survey.

    Endocrine connections·2024
    Same author

    Controlled Growth of the Self-Modulation of a Relativistic Proton Bunch in Plasma.

    Physical review letters·2022
    Same author

    Rationale and design of the multicentric, double-blind, double-placebo, randomized trial APrepitant versus HYdroxyzine in association with cytoreductive treatments for patients with myeloproliferative neoplasia suffering from Persistent Aquagenic Pruritus. Trial acronym: APHYPAP.

    Trials·2021
    Same author

    Haematological parameters associated with postpartum haemorrhage after vaginal delivery: Results from a French cohort study.

    Journal of gynecology obstetrics and human reproduction·2021
    Same journal

    Comparison of expression patterns and cell adhesion properties of the mouse biliary glycoproteins Bgp1 and Bgp2.

    European journal of biochemistry·2020
    Same journal

    AB 3.1.1.1 (or EC 3.1.1.?).

    European journal of biochemistry·2020
    Same journal

    Cdk5.

    European journal of biochemistry·2018
    Same journal

    Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola.

    European journal of biochemistry·2004
    Same journal

    Monitoring ligand-mediated nuclear receptor-coregulator interactions by noncovalent mass spectrometry.

    European journal of biochemistry·2004
    Same journal

    Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy.

    European journal of biochemistry·2004
    See all related articles

    Chicken gizzard actin preparations can be contaminated with beta-actinin. Purified gizzard actin polymerization is similar to skeletal muscle actin, but Ca2+ binding is weaker.

    Area of Science:

    • Biochemistry
    • Muscle Physiology

    Background:

    • Actin is a crucial protein in muscle contraction.
    • Chicken gizzard actin preparations may contain contaminants affecting properties.

    Purpose of the Study:

    • To investigate the purification of chicken gizzard actin.
    • To characterize the properties of purified gizzard actin.

    Main Methods:

    • Acetone-dried muscle powder preparation.
    • EDTA washing and Sephadex G-100 gel filtration.
    • Actin polymerization and ATP-splitting activity assays.

    Main Results:

    • Beta-actinin contamination reduced in EDTA-washed preparations.
    • Sephadex G-100 gel filtration removed beta-actinin.

    Related Experiment Videos

  • Purified gizzard actin polymerization matched skeletal muscle actin.
  • Gizzard actin G-form showed Ca2+ instability, indicating lower affinity.

    • Conclusions:

    • EDTA washing and gel filtration effectively purify gizzard actin.
    • Gizzard actin polymerization is similar to skeletal muscle actin.
    • Gizzard actin has a lower Ca2+ affinity compared to skeletal muscle actin.