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Genetic mapping in phage group 2 Staphylococcus aureus.

S M Martin, S C Shoham, M Alsup

    Infection and Immunity
    |February 1, 1980
    PubMed
    Summary
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    Researchers developed transformation methods for Staphylococcus aureus strains. Genetic mapping identified linkage groups on both strains, but the exfoliative toxin gene

    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Staphylococcus aureus strains UT0002-19 and UT0017 are used for genetic studies.
    • Strain UT0002-19 produces exfoliative toxin, causing staphylococcal scalded skin syndrome.
    • Strain UT0017 lacks exfoliative toxin production.

    Purpose of the Study:

    • To develop transformation regimens for Staphylococcus aureus strains UT0002-19 and UT0017.
    • To genetically map chromosomal markers in these strains.
    • To investigate the genetic basis of exfoliative toxin production.

    Main Methods:

    • Isolation of antibiotic-resistant and auxotrophic mutants using gradient plates, trimethoprim selection, and nitrosoguanidine mutagenesis.
    • Transformation of mutant strains to determine genetic marker frequencies.

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  • Genetic linkage analysis to identify chromosomal groups.

    • Main Results:

    • Transformation frequencies ranged from 10(-6) to 10(-8).
    • Three genetic linkage groups were identified on strain UT0017: thy-4-lys-5-trp-21-thr-4, pyr-26-nov-9-his-3, and ilv-9-pen-1.
    • Two genetic linkage groups were identified on strain UT0002-19: thy-1-lys-5-trp-3-thr-4-ala-8 and nov-9-his-3.
    • A specific chromosomal locus for exfoliative toxin synthesis was not mapped.

    Conclusions:

    • Effective transformation protocols were established for Staphylococcus aureus.
    • The study elucidated chromosomal organization and genetic linkage in two distinct strains.
    • The genetic basis of exfoliative toxin production remains unmapped in these strains.