Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitochondrial proliferation in cardiac hypertrophy.

R Zak, M Rabinowitz, C Rajamanickam

    Basic Research in Cardiology
    |January 1, 1980
    PubMed
    Summary

    Mitochondrial DNA (mtDNA) increased significantly in rat hearts after aortic constriction, suggesting mtDNA replication is crucial for cardiac adaptation. This study reveals that mtDNA availability isn't the sole regulator of mitochondrial gene expression during cardiac hypertrophy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A phase II study of pembrolizumab plus chemotherapy with short-course radiotherapy as induction treatment for unresectable locally advanced esophageal squamous cell carcinoma: PALADIN trial.

    ESMO gastrointestinal oncology·2026
    Same author

    Survival benefit of adjuvant chemotherapy based on molecular residual disease detection in resected colorectal liver metastases: subgroup analysis from CIRCULATE-Japan GALAXY.

    Annals of oncology : official journal of the European Society for Medical Oncology·2024
    Same author

    Processed electroencephalography: impact of patient age and surgical position on intraoperative processed electroencephalogram monitoring of burst-suppression.

    Journal of clinical monitoring and computing·2021
    Same author

    Vaccine implementation factors affecting maternal tetanus immunization in low- and middle-income countries: Results of the Maternal Immunization and Antenatal Care Situational Analysis (MIACSA) project.

    Vaccine·2020
    Same author

    Antenatal care service delivery and factors affecting effective tetanus vaccine coverage in low- and middle-income countries: Results of the Maternal Immunisation and Antenatal Care Situational analysis (MIACSA) project.

    Vaccine·2020
    Same author

    Magnetic-Field-Induced Suppression of Jahn-Teller Phonon Bands in (La<sub>0.6</sub>Pr<sub>0.4</sub>)<sub>0.7</sub>Ca<sub>0.3</sub>MnO<sub>3</sub>: the Mechanism of Colossal Magnetoresistance shown by Raman Spectroscopy.

    Scientific reports·2019

    Area of Science:

    • Cardiovascular Biology
    • Mitochondrial Biology
    • Molecular Cardiology

    Background:

    • Cardiac hypertrophy, a response to increased workload, involves complex cellular adaptations.
    • Mitochondria play a critical role in energy production and cellular signaling within cardiomyocytes.

    Purpose of the Study:

    • To investigate mitochondrial DNA (mtDNA) proliferation during cardiac hypertrophy induced by aortic constriction in mature female rats.
    • To elucidate the relationship between mtDNA replication and the accumulation of mitochondrial proteins.

    Main Methods:

    • Aortic constriction was performed on mature female rats to induce cardiac hypertrophy.
    • Mitochondrial DNA (mtDNA) concentration was quantified using a fluorometric method after nuclear DNA removal.
    • Cytochrome c, b, and aa3 accumulation rates were measured and compared to mtDNA levels.

    Related Experiment Videos

  • Replicative intermediates of mtDNA, including D-loops, were analyzed.

    • Main Results:

    • Mitochondrial DNA (mtDNA) concentration increased by 11%, 72%, and 117% at 2, 4, and 7 days post-surgery, respectively.
    • Initially, inner mitochondrial components accumulated faster than mtDNA, but mtDNA replication outpaced cytochrome accumulation in later stages.
    • A significant decrease in D-loop frequency was observed in hypertrophied hearts, indicating a removal of a replication block.

    Conclusions:

    • Mitochondrial DNA (mtDNA) replication is a key component of the adaptive response to cardiac hypertrophy.
    • The rate of mtDNA accumulation is not solely dictated by template availability, suggesting other regulatory mechanisms.
    • Enhanced mtDNA replication during hypertrophy involves overcoming a block in the conversion of D-loop intermediates.