Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The rate-limiting step in muscle contraction.

R J Podolsky

    Basic Research in Cardiology
    |January 1, 1980
    PubMed
    Summary

    This study investigated muscle fiber motion to understand the cross-bridge cycle. Results suggest muscle contraction rate is limited by myosin transitions, not cross-bridge cycling itself.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The structure of water and electrolyte solutions.

    Lecture and review series. Naval Medical Research Institute (U.S.)·2014
    Same author

    Isometric tension and mutant myosin heavy chain content in single skeletal myofibers from hypertrophic cardiomyopathy patients.

    Journal of molecular and cellular cardiology·1997
    Same author

    Crossbridge rotation in EDC-crosslinked striated muscle fibers.

    Advances in experimental medicine and biology·1993
    Same author

    X-ray diffraction testing for weak-binding crossbridges in relaxed bony fish muscle fibres at low ionic strength.

    Journal of structural biology·1991
    Same author

    Single skinned muscle fibers in Duchenne muscular dystrophy generate normal force.

    Annals of neurology·1990
    Same author

    Elastic behavior of connectin filaments during thick filament movement in activated skeletal muscle.

    The Journal of cell biology·1989

    Area of Science:

    • Muscle physiology
    • Biophysics
    • Biochemistry

    Background:

    • The cross-bridge cycle is fundamental to muscle contraction.
    • Understanding the factors limiting contraction speed is crucial for muscle research.

    Purpose of the Study:

    • To investigate the cross-bridge cycle kinetics in skinned muscle fibers.
    • To determine the effects of ATP regeneration and ionic strength on muscle fiber motion.

    Main Methods:

    • Studied skinned muscle fibers in controlled chemical solutions.
    • Applied step changes in load and analyzed transient and steady responses.
    • Varied ATP regenerating system and ionic strength.

    Main Results:

    • Changes in ATP regeneration and ionic strength affected the transient response of muscle fibers.
    • The steady-state motion of muscle fibers remained unaffected by these changes.
    • Transient response alterations suggest modifications in cross-bridge attachment and detachment rates.

    Conclusions:

    • The rate of physiological muscle contraction is likely limited by a slow transition within the unattached myosin molecule.
    • This finding differentiates the rate-limiting step from the cross-bridge making/breaking process itself.
    • Provides new insights into the molecular mechanisms governing muscle contraction speed.

    Related Experiment Videos