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[Insulin receptors in dog lymphocytes]
Comptes Rendus Des Seances De La Societe De Biologie Et De Ses Filiales
|January 1, 1980
Summary
Dog lymphocytes serve as a model for studying insulin receptors. Researchers found fewer insulin receptors in diabetic dogs, suggesting a link to diabetes pathology.
Area of Science:
- Endocrinology
- Immunology
- Veterinary Medicine
Context:
- Circulating lymphocytes are easily accessible for studying insulin receptors in dogs.
- Insulin receptor studies are crucial for understanding metabolic disorders.
- Ficoll gradient preparation yields lymphocytes suitable for binding assays.
Purpose:
- To investigate the binding characteristics of insulin to dog lymphocytes.
- To quantify insulin receptors on canine lymphocytes.
- To explore potential alterations in insulin receptor number in diabetic dogs.
Summary:
- Circulating dog lymphocytes effectively bind radio-iodinated insulin (125I-Insulin), with binding being dependent on cell and insulin concentrations.
- Binding reached equilibrium within 30 minutes at 4°C, and Scatchard analysis indicated approximately 1000-1500 receptors per cell.
- A reduced number of insulin binding sites was observed in lymphocytes from diabetic dogs.
Impact:
- This research establishes lymphocytes as a viable model for canine insulin receptor studies.
- Findings suggest a potential role for lymphocyte insulin receptors in understanding canine diabetes.
- Provides a foundation for further research into insulin resistance and diabetes in veterinary medicine.