Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Abnormal B cell differentiation and variable increased T cell suppression in immunodeficiency with hyper-IgM.

H J Krantman, E R Stiehm, R H Stevens

    Clinical and Experimental Immunology
    |April 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Risk Factors Associated with HCV Among Opioid-Dependent Patients in a Multisite Study.

    Journal of community health·2015
    Same author

    Prevention of oral food allergy sensitization via skin application of food allergen in a mouse model.

    Allergy·2012
    Same author

    What impact will the immune tolerance network have on the future treatment of allergic diseases?

    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2004
    Same author

    Breast milk transmission of viral disease.

    Advances in nutritional research·2002
    Same author

    Juvenile idiopathic polyarticular arthritis and IgA deficiency in the 22q11 deletion syndrome.

    The Journal of rheumatology·2001
    Same author

    Cytokine and chemokine dysregulation in hyper-IgE syndrome.

    Clinical immunology (Orlando, Fla.)·2001

    Patients with hyper-IgM immunodeficiency show defective B cell differentiation into IgG- and IgA-producing cells. Some also exhibit excessive T suppressor activity, hindering normal immunoglobulin synthesis.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Hyper-IgM immunodeficiency is a rare primary immunodeficiency characterized by normal or elevated IgM levels with severely decreased IgG, IgA, and IgE.
    • The precise mechanisms underlying B cell dysfunction and the role of T cells in this condition are not fully elucidated.

    Purpose of the Study:

    • To investigate the in vitro immunoglobulin (Ig) synthesis capacity of B cells and T cells from patients with hyper-IgM immunodeficiency.
    • To determine the potential role of T cell regulatory abnormalities in the pathogenesis of hyper-IgM.

    Main Methods:

    • Separation and co-culture of patient and normal T and B cells.
    • Measurement of de novo immunoglobulin synthesis using 35S-methionine incorporation.
    • Determination of immunoglobulin classes by SDS-PAGE and autoradiography.

    Related Experiment Videos

    Main Results:

    • Patient B cells produced only IgM, irrespective of T cell source (autologous or allogeneic).
    • Normal B cells produced IgM, IgG, and IgA when co-cultured with normal T cells.
    • T cells from two patients suppressed normal B cell Ig synthesis; irradiation restored T helper function.
    • T cells from the third patient displayed normal suppressor/helper activity.

    Conclusions:

    • Defective B cell differentiation into IgG- and IgA-producing plasma cells is a consistent feature of hyper-IgM immunodeficiency.
    • Excessive T suppressor cell activity is a variable, but potentially significant, accompanying abnormality in some patients.