Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein utilization in correlation to energy intake.

M Krajcovicová, O Dibák

    Physiologia Bohemoslovaca
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Physiologic requirements for casein protein and wheat gluten in 4-month-old rats].

    Veterinarni medicina·1990
    Same author

    [Utilization of proteins in relation to age and intake of fats and saccharides].

    Bratislavske lekarske listy·1988
    Same author

    Age-correlation of protein utilization to saccharide intake.

    Physiologia Bohemoslovaca·1988
    Same author

    [Protein retention in relation to age].

    Veterinarni medicina·1988
    Same author

    Body composition and physiological casein and wheat gluten protein requirements of 180-day-old rats.

    Physiologia Bohemoslovaca·1986
    Same author

    The relationship between protein retention and energy requirements in rats of different ages.

    Physiologia Bohemoslovaca·1986

    The optimal diet for weaned rats includes 10% protein and 30% fat, promoting growth and protein utilization. Higher fat content (35-40%) negatively impacts metabolic processes and protein value.

    Area of Science:

    • Nutritional Biochemistry
    • Animal Physiology
    • Metabolic Studies

    Background:

    • Understanding optimal nutrient balance is crucial for early-life animal development.
    • Dietary fat and energy intake significantly influence protein utilization and metabolic pathways.
    • Assessing biological protein value requires evaluating growth and key liver metabolic processes.

    Purpose of the Study:

    • To determine the optimal dietary fat and energy levels for weaned rats on a fixed protein intake.
    • To investigate the impact of varying fat quantities on protein utilization parameters (PER, NPR, NPU, LPU).
    • To analyze the effects of dietary fat on hepatic metabolic pathways including gluconeogenesis, glycolysis, pentose cycle, citric acid cycle, and transamination.

    Main Methods:

    • Weaned rats (60g) were fed diets with varying fat and energy levels for 14 days.

    Related Experiment Videos

  • Evaluated growth and biological protein value using Protein Efficiency Ratio (PER), Net Protein Ratio (NPR), and Net Protein Utilization (NPU) for body and Liver Protein Utilization (LPU).
  • Assessed liver metabolic activity, including gluconeogenesis, glycolysis, pentose cycle, citric acid cycle, and transamination.

    • Main Results:

    • A diet of 10% protein, 30% fat (2.274 MJ) yielded maximum PER, NPR, NPU, and LPU values.
    • Higher fat content (35-40%) stimulated gluconeogenesis and transamination.
    • High fat diets inhibited glycolysis, the citric acid cycle, and lipogenesis, reducing protein biological value parameters.

    Conclusions:

    • The ideal diet for newly weaned rats consists of 10% high-quality protein, 30% fat, and 2.274 MJ.
    • Excessive dietary fat negatively affects protein utilization and disrupts key hepatic metabolic pathways.
    • Optimizing macronutrient ratios is essential for maximizing growth and metabolic health in young animals.