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Related Experiment Videos

Functional differences between cells from MLR colonies grown in soft agar cultures.

A Zeevi, K M Chiu, R J Duquesnoy

    Journal of Immunology (Baltimore, Md. : 1950)
    |September 1, 1980
    PubMed
    Summary

    This study introduces a novel soft agar colony assay to grow human T lymphocytes activated in mixed lymphocyte reactions (MLR). The assay successfully differentiates T cell subpopulations with distinct cytotoxic and suppressive activities, aiding in MLR research.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Mixed lymphocyte reaction (MLR) is crucial for studying T cell activation.
    • Differentiating functional T lymphocyte subpopulations is essential for understanding immune responses.

    Purpose of the Study:

    • To develop and characterize a soft agar colony assay for human T lymphocytes activated in MLR.
    • To investigate the functional properties of distinct T lymphocyte subpopulations generated by this assay.

    Main Methods:

    • Activation of human T lymphocytes in MLR with specific priming and T cell growth factors.
    • Culturing activated lymphocytes in soft agar to form distinct colony types (upper and lower).
    • Assessing cytotoxic (CML) and suppressor cell activity of lymphocytes derived from these colonies.

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    Main Results:

    • Two distinct T lymphocyte colony types (upper and lower) were identified in soft agar.
    • Upper colony cells exhibited significantly higher cell-mediated lympholysis (CML) activity compared to lower colony cells.
    • Both colony types possessed radiosensitive suppressor cell activity, with differing specificities.

    Conclusions:

    • The soft agar colony assay is a promising method for separating and analyzing functionally distinct T lymphocyte subpopulations from MLR cultures.
    • The assay facilitates the study of T cell subsets with varying cytotoxic and regulatory functions.