Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Standardization of flow microfluorometers using glutaraldehyde fixed lymphocytes.

R A Zbroja, J Wass, P C Vincent

    Pathology
    |July 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The comparative efficacy of theta burst stimulation or functional electrical stimulation when combined with physical therapy after stroke: a randomized controlled trial.

    Clinical rehabilitation·2019
    Same author

    SOCIETY FOR ENDOCRINOLOGY CLINICAL GUIDANCE: Inpatient management of cranial diabetes insipidus.

    Endocrine connections·2018
    Same author

    Blood Volume in Malignant Disease: Case Studies in 86 Females1.

    The Australian and New Zealand journal of surgery·2017
    Same author

    Commissioning guidance for weight assessment and management in adults and children with severe complex obesity.

    Obesity reviews : an official journal of the International Association for the Study of Obesity·2017
    Same author

    Late Relapses in Hodgkin's Disease: Are They a Distinct Entity?

    Leukemia & lymphoma·2016
    Same author

    Developing a specialist obesity infrastructure: an example from current strategies in England.

    Clinical obesity·2015

    Glutaraldehyde-fixed lymphocytes offer a stable, reproducible reference standard for calibrating flow microfluorometers. This method reliably positions cell cycle peaks and aids in detecting aneuploid cell populations.

    Area of Science:

    • Cell biology
    • Biotechnology
    • Analytical chemistry

    Background:

    • Flow microfluorometry requires reliable calibration standards for accurate cell analysis.
    • Existing standards may lack stability or reproducibility for precise measurements.
    • Accurate G1 peak determination is crucial for cell cycle analysis and ploidy assessment.

    Purpose of the Study:

    • To describe the preparation of glutaraldehyde-fixed lymphocytes as a reference standard for flow microfluorometry.
    • To evaluate the suitability of these fixed cells for instrument calibration and aneuploidy detection.

    Main Methods:

    • Lymphocytes were fixed using glutaraldehyde.
    • The stability and staining reproducibility of fixed cells were assessed over time.
    • Fixed cells were used to determine the G1 peak of normal mammalian cells.

    Related Experiment Videos

    Main Results:

    • Glutaraldehyde-fixed lymphocytes are easy to prepare and demonstrate long-term stability.
    • These cells stain reproducibly, aligning with expected values for fresh human cells.
    • The fixed cells effectively resolve and correctly situate the G1 peak for mammalian cells.

    Conclusions:

    • Glutaraldehyde-fixed lymphocytes serve as a reliable reference standard for flow microfluorometer calibration.
    • This method facilitates accurate detection of aneuploid cell populations.
    • The preparation offers a stable and reproducible tool for cytometric analysis.