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Updated: Sep 13, 2026

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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Published on: March 16, 2011
An easy method for the selection of restriction- and modification-deficient mutants of Escherichia coli K-12
Gene
|October 1, 1980
Abstract:
An easy and rapid method for selecting restriction- and modification-defective mutants of Escherichia coli K-12 is described. This method employs selection of tetracycline resistant lysogens after infection with lambda::Tn10 phage and results in a high yield of spontaneous rk-mk- and rk-mk+ mutants.

