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Updated: Aug 19, 2026

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress
Published on: March 21, 2014
[Modification of leucocyte adherence inhibition (LAI) assay with 51Cr-labelled leucocytes (author's transl)]
Leucocyte adherence inhibition was measured by a new modified radioisotopic technique. Peripheral blood leucocytes were isolated and labelled with Chrom51 (51Cr). These leucocytes were incubated with medium of buffer alone and with medium or buffer containing tumor antigen or gluten. The glass surface for the adherence was prepared carefully. At all samples the adherence of leucocytes occurred under the same conditions. The authors gave a report on the results of their leucocyte adherence inhibition (LAI) assay with gluten and stomach cancer antigen.
Leucocyte adherence inhibition was measured by a new modified radioisotopic technique. Peripheral blood leucocytes were isolated and labelled with Chrom51 (51Cr). These leucocytes were incubated with medium of buffer alone and with medium or buffer containing tumor antigen or gluten. The glass surface for the adherence was prepared carefully. At all samples the adherence of leucocytes occurred under the same conditions. The authors gave a report on the results of their leucocyte adherence inhibition (LAI) assay with gluten and stomach cancer antigen.

