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Related Experiment Videos

Screening of carcinogens with the prophage lambda cIts857 induction test.

Y L Ho, S K Ho

    Cancer Research
    |February 1, 1981
    PubMed
    Summary

    This study introduces a sensitive prophage lambda induction test for screening sparingly soluble carcinogens. The enhanced test demonstrates improved correlation with carcinogenicity compared to traditional methods, offering a faster and economical alternative.

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    Area of Science:

    • Molecular Biology
    • Toxicology
    • Genetics

    Background:

    • Screening for carcinogenicity is crucial for public health.
    • Existing methods like the Ames test have limitations, especially with sparingly soluble compounds.
    • Prophage induction assays offer a potential alternative for carcinogen detection.

    Purpose of the Study:

    • To evaluate the efficacy of the prophage lambda cIts857 induction test for screening sparingly water-soluble carcinogens.
    • To assess the test's sensitivity and correlation with known carcinogenicity, particularly in comparison to the Ames test.
    • To optimize the induction test procedure for speed and economy.

    Main Methods:

    • Utilized the prophage lambda cIts857 induction test with Escherichia coli K12 envA uvrB lysogen.
    • Dissolved sparingly soluble carcinogens in dimethyl sulfoxide and activated them metabolically using liver enzymes.
    • Suppressed growth of noninduced cells with ampicillin to enhance test sensitivity.
    • Employed the endolysin assay technique for simplified and rapid assessment of prophage induction.

    Main Results:

    • The lambda cIts857 induction test successfully screened sparingly soluble carcinogens requiring metabolic activation.
    • The test demonstrated high sensitivity, with maximum induction activity reaching 50% of that for water-soluble carcinogens.
    • The induction test showed better correlation with carcinogenicity than the Ames test in several cases, with only one false negative (cyclophosphamide).
    • The wild-type prophage lambda strain yielded low sensitivity.

    Conclusions:

    • The prophage lambda cIts857 induction test is a sensitive and effective method for screening sparingly soluble carcinogens.
    • This assay provides a valuable alternative or complementary tool to existing carcinogenicity tests, offering improved correlation and efficiency.
    • The use of the endolysin assay simplifies the procedure, making the screening test quick and economical.

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