Binding of phencyclidine to rat brain membranes: technical aspect

[3H]Phencyclidine has a tendency to adsorb to filters used in binding experiments. The spinal binding techniques that should be used to overcome this difficulty are described. The affinity of [3H]phencyclidine for its brain receptor was 4 times higher in a medium containing 5 mM salt than in a medium containing 50 mM salt.