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[Standardization of indirect immunofluorescence for detection of autoantibodies (author's transl)]
Seven laboratories made a chessboard titration with a serum containing antimitochondrial antibodies and two conjugates labeled with fluorescein isothiocyanate. The variation of the plateau titer and the titer of the serum as well as the plateau-end point was slight with the exception of one case. The standardization of indirect immunofluorescence needs a close contact between the laboratories, the availability of reference sera and conjugates, and similar microscopic equipment.
Seven laboratories made a chessboard titration with a serum containing antimitochondrial antibodies and two conjugates labeled with fluorescein isothiocyanate. The variation of the plateau titer and the titer of the serum as well as the plateau-end point was slight with the exception of one case. The standardization of indirect immunofluorescence needs a close contact between the laboratories, the availability of reference sera and conjugates, and similar microscopic equipment.