Nucleases and their control in wild-type and nuh mutants of Neurospora

A review of all of the work on Neurospora nucleases strongly suggests that five nucleases, originally isolated on the basis of markedly different properties, may actually be derived from a single inactive precursor polypeptide via different routes of proteolysis. One of these nucleases may be involved in DNA repair and/or recombination. Two repair-deficient mutants of Neurospora, uvs-3 and nuh-4, may have a lesion in protease(s) which control the level of this nuclease or in some function which regulates the protease(s). Both of these mutants map in the same gene region and they may be defective in recombination, since they are sensitive to various mutagens and to mitomycin C and they show high frequency of spontaneous, but not radiation-induced, recessive lethal mutations and/or deletions.