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Canine surface active material and pulmonary lymphocyte function. Studies with mixed-lymphocyte culture
Experimental Lung Research
|March 1, 1980
Summary
Canine pulmonary lymphocytes show reduced responsiveness in mixed lymphocyte culture (MLC) but can stimulate blood lymphocytes. Exposure to lung surfactant (SAM) in vitro mimics this immune behavior, suggesting SAM
Area of Science:
- Immunology
- Pulmonary Medicine
- Cellular Biology
Background:
- Bronchoalveolar lavage yields pulmonary lymphocytes with distinct immune functions.
- Circulating blood lymphocytes typically exhibit robust responses in mixed lymphocyte culture (MLC).
Purpose of the Study:
- To investigate the immune function of canine pulmonary lymphocytes in MLC.
- To determine the effect of canine surface active material (SAM) on lymphocyte function.
Main Methods:
- Enrichment of canine bronchoalveolar lymphocytes.
- Testing lymphocyte responsiveness and stimulatory capacity in MLC.
- In vitro exposure of blood lymphocytes to SAM.
Main Results:
- Pulmonary lymphocytes demonstrated significantly lower responsiveness to allogeneic stimulation in MLC compared to blood lymphocytes.
- Pulmonary lymphocytes retained the ability to stimulate allogeneic blood lymphocytes.
- In vitro exposure to SAM suppressed blood lymphocyte responsiveness but minimally affected their stimulatory capacity.
Conclusions:
- Pulmonary lymphocytes exhibit hyporesponsiveness and stimulatory capacity, differentiating them from blood lymphocytes.
- SAM exposure in vitro converts blood lymphocytes to a state mimicking pulmonary lymphocytes.
- In vivo SAM exposure may underlie the altered immune reactivity of pulmonary lymphocytes.