Isozymes in wheat-barley hybrid derivative lines

Zymogram analysis was used to identify the barley chromosomes that carry the structural genes for particular isozymes. Wheat, barley, and wheat-barley hybrid derivative lines (which contained identified barley chromosomes) were tested by gel electrophoresis for isozymes of particular enzymes. It was found that barley chromosome 4 carries structural genes for acid phosphatase and beta amylase isozymes, barley chromosome 5 carries genes for phosphoglucose isomerase and male dehydrogenase isozymes, and that barley chromosome 2 carries a gene for at least one glucose-6-phosphate dehydrogenase protomer. These results reinforce previous conclusions that barley chromosome 4 shows homoeology with wheat chromosome group 4 and that barley chromosome 5 shows homoeology with wheat chromosome group 1.