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Related Experiment Videos

Human suppressor cells: evidence of functionally distinct subsets.

N E Goeken, J S Thompson, L Klassen

    Human Immunology
    |February 1, 1981
    PubMed
    Summary

    Human suppressor cells (SC) exhibit distinct differentiation pathways. Concanavalin A (Con A) induces early, non-specific SC, while allogeneic stimulation generates later, specific SC, suggesting two distinct human suppressor cell subsets.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Human suppressor cells (SC) play a critical role in immune regulation.
    • SC can be induced through various stimuli, exhibiting different characteristics.

    Purpose of the Study:

    • To investigate the differentiation pathways and characteristics of human suppressor cells induced by Concanavalin A (Con A) and allogeneic stimulation.
    • To elucidate the distinct properties and subsets of human suppressor cells.

    Main Methods:

    • Induction of SC in vitro using Concanavalin A (Con A) or repeated allogeneic stimulation.
    • Coculture experiments with autologous cells and analysis of blastogenesis.
    • Treatment with 5-bromodeoxyuridine and light, mitomycin C, and theophylline to characterize cell subsets.

    Main Results:

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    • Con A-induced SC showed early blastogenesis and rapid suppression, mediated by mitomycin C-sensitive T cells.
    • Allogeneic stimulation induced mitomycin C-resistant SC, suppressive in MLC but not Con A cultures.
    • These findings suggest at least two distinct human SC subsets: a non-specific, early-acting cell and a specific, later-acting effector cell.

    Conclusions:

    • Human suppressor cells differentiate into at least two distinct subsets with differing kinetic and specificity profiles.
    • One subset is mitomycin-sensitive and non-specific, involved in early events.
    • A second subset is mitomycin-resistant and appears to acquire specificity for immune responses.