Related Experiment Videos
Separation of ATP-dependent DNAse to ATPase and DNAse
Acta Biologica Academiae Scientiarum Hungaricae
|January 1, 1981
Abstract:
A 250-fold purified ATP-dependent DNase from Bacillus cereus has been separated to DNA-dependent ATPase I and II and a DNase specific for single-stranded DNA (ssDNase) by means of high resolution of DEAE cellulose chromatography. Simultaneously with the separation of ATPase and ssDNase, a decrease in ATP-dependent DNase activity was observed. Complete separation resulted in the total loss of ATP-dependent DNase activity. Reconstitution of ATP-stimulated DNase activity was dependent on the ratio of the combined ATPase II and ssDNase.