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Related Experiment Videos

Cell lysis by induction of cloned lambda lysis genes.

J Garrett, R Fusselman, J Hise

    Molecular & General Genetics : MGG
    |January 1, 1981
    PubMed
    Summary

    Bacteriophage lambda lysis genes S, R, and Rz were cloned and expressed. Premature lysis is an S-dependent phenomenon, controlled by the S gene product interacting with a host anti-S protein.

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    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Bacteriophage lambda is a model organism for studying viral replication and lysis.
    • The lysis gene region (S, R, Rz) controls the final stages of phage release.
    • Understanding lysis control is crucial for phage therapy and genetic engineering.

    Purpose of the Study:

    • To clone and express the bacteriophage lambda lysis genes (S, R, Rz) in a plasmid system.
    • To investigate the role of the S gene in bacteriophage lambda-induced lysis.
    • To elucidate the mechanism controlling lysis timing.

    Main Methods:

    • Cloning of bacteriophage lambda lysis genes into the pBH20 plasmid.
    • Expression of the lysis operon under the control of the lac promoter (lacOP).
    • Induction using IPTG and assessment of lysis timing and bacteriolytic activity.

    Main Results:

    • Recombinant plasmid with lysis genes showed sharply defined lysis after 35 min upon induction.
    • Premature lysis was induced by cyanide, chloramphenicol, or chloroform.
    • Induction of an S- mutant lysis operon led to intracellular bacteriolytic activity accumulation without lysis.
    • S- mutant clones were insensitive to premature lysis inducers.

    Conclusions:

    • Premature lysis in bacteriophage lambda infections is an S-dependent phenomenon.
    • A model is proposed where lysis timing is determined by the accumulation of the S gene product competing with a host anti-S protein.

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